Fatty acids in Krestyanskoe butter

• Column:
  Synergi Fusion-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard Fusion-RP 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  acetonitrile / water / acetic acid (110:12:3)
• Flow rate:
  0.9 ml / min
• Column temperature:
  20 °С
• Sample volume:
  10 µl
• Detector:
  Low temperature evaporative light scattering detector
• Detection parameters:
  evaporating tube temperature 40 °C, gain - 1, pressure - 3 bar
• Object of study:
  Dairy products, butter, cheese
• Substance groups:
  Fatty acids
• Sample:
  Peasant butter
• Methodology:
  Methods for measuring the mass fraction of fatty acids in animal and vegetable fats and oils, margarines, fats for cooking, confectionery and bakery industries by high performance liquid chromatography