Amino acids in compound feed

• Column:
  Synergi Fusion-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard Fusion-RP 4х3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 25 mM sodium acetate trihydrate solution in water
  B: acetonitrile
• Flow rate:
  1.0 ml / min
• Column temperature:
  55 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 436 nm
• Object of study:
  Compound feed and raw materials
• Substance groups:
  Amino acids
• Sample:
  compound feed (derivatization of DABS-Cl)
• Methodology:
  Methods for measuring the mass fraction of lysine, tryptophan, methionine, the sum of cystine and cysteine in compound feed, premixes and compound feed raw materials by high performance liquid chromatography